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Published 07:08 3 Oct 2016 BST
Updated 16:15 3 Oct 2016 BST
"It was a gruelling affair," says Stuart Phillips, a professor in the Department of Kinesiology at McMaster and senior investigator on the study.
"These guys were in rough shape, but that was part of the plan. We wanted to see how quickly we could get them into shape: lose some fat, but still retain their muscle and improve their strength and fitness."
The men were divided into two groups - both on the low calorie diet, but one with higher levels of protein that the other.
The higher intake group were on 2.4g of protein per kg of bodyweight everyday while the lower group were on half that.
One major finding after four weeks was that the higher protein group added 2.5lbs of muscle, despite consuming not nearly enough energy from their diet.
The lower protein group did not add any muscle, although they did not lose any muscle either.
"Exercise, particularly lifting weights, provides a signal for muscle to be retained even when you're in a big calorie deficit," says Phillips.
[caption id="attachment_58527" align="alignnone" width="507"]
Young male bodybuilder doing heavy weight exercise[/caption]
But what researchers were intrigued by was that the high protein group also lost more body fat.
The group lost about 10.5lbs of fat on average while the low protein group lost more than 20% less.
"We expected the muscle retention" said Phillips, "but were a little surprised by the amount of additional fat loss in the higher protein consuming group."
What was clear was that all the men on the study became stronger, fitter and in much better shape because of the demanding six-days-a-week exercise programme.
There are some caveats to the research and Phillips explained "We designed this program for overweight young men, although I'm sure it would work for young women too, to get fitter, stronger, and to lose weight fast.
"It's a tough programme and not something that's sustainable or for those looking for quick and easy fix.
"We controlled their diets, we supervised the exercise, and we really kept these guys under our 'scientific' thumb for the four weeks the participants were in the study."
So the bottom line was a high intake of protein while eating a low-calorie diet 40% lower than the recommended amount, combined with some hardcore six-day-a-week weights and interval training was effective at maintaining lean body mass and burning fat.
[caption id="attachment_58528" align="alignnone" width="725"]
Lifting weights[/caption]

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